Best practices for the extraction of genomic DNA from formalin?fixed paraffin?embedded tumor tissue for cancer genomic profiling tests

Recently, two cancer genomic profiling tests have been approved in Japan and implemented routine clinical practice: the FDA-approved FoundationOne CDx test, OncoGuide NCC Oncopanel test. The quality quantity of DNA significantly affects sequencing results; therefore, preparing a sufficient amount high-quality for is important. We examined best practices extraction from formalin-fixed paraffin-embedded (FFPE) tumor tissues pancreatic, lung colon specimens. found that extracted 10-?m-thick FFPE samples improved significantly, compared with 4-?m-thick samples, suggesting are preferable tests. For convenience, we created quick reference table calculating required number slides.